                         SEQUENCE LISTING

<110>  Fondazione Luigi Maria Monti
       Universit degli Studi di Roma "Tor Vergata"
 
<120>  anti-VEGFR-1 antibodies and uses thereof

<130>  132582 PCT

<150>  IT 102016000034933
<151>  2016-04-05

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  687
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Ser Tyr Trp Asp Thr Gly Val Leu Leu Cys Ala Leu Leu Ser 
1               5                   10                  15      


Cys Leu Leu Leu Thr Gly Ser Ser Ser Gly Ser Lys Leu Lys Asp Pro 
            20                  25                  30          


Glu Leu Ser Leu Lys Gly Thr Gln His Ile Met Gln Ala Gly Gln Thr 
        35                  40                  45              


Leu His Leu Gln Cys Arg Gly Glu Ala Ala His Lys Trp Ser Leu Pro 
    50                  55                  60                  


Glu Met Val Ser Lys Glu Ser Glu Arg Leu Ser Ile Thr Lys Ser Ala 
65                  70                  75                  80  


Cys Gly Arg Asn Gly Lys Gln Phe Cys Ser Thr Leu Thr Leu Asn Thr 
                85                  90                  95      


Ala Gln Ala Asn His Thr Gly Phe Tyr Ser Cys Lys Tyr Leu Ala Val 
            100                 105                 110         


Pro Thr Ser Lys Lys Lys Glu Thr Glu Ser Ala Ile Tyr Ile Phe Ile 
        115                 120                 125             


Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
    130                 135                 140                 


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
145                 150                 155                 160 


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
                165                 170                 175     


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
            180                 185                 190         


Ile Ile Ser Asn Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
        195                 200                 205             


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
    210                 215                 220                 


Gln Thr Asn Thr Ile Ile Asp Val Gln Ile Ser Thr Pro Arg Pro Val 
225                 230                 235                 240 


Lys Leu Leu Arg Gly His Thr Leu Val Leu Asn Cys Thr Ala Thr Thr 
                245                 250                 255     


Pro Leu Asn Thr Arg Val Gln Met Thr Trp Ser Tyr Pro Asp Glu Lys 
            260                 265                 270         


Asn Lys Arg Ala Ser Val Arg Arg Arg Ile Asp Gln Ser Asn Ser His 
        275                 280                 285             


Ala Asn Ile Phe Tyr Ser Val Leu Thr Ile Asp Lys Met Gln Asn Lys 
    290                 295                 300                 


Asp Lys Gly Leu Tyr Thr Cys Arg Val Arg Ser Gly Pro Ser Phe Lys 
305                 310                 315                 320 


Ser Val Asn Thr Ser Val His Ile Tyr Asp Lys Ala Phe Ile Thr Val 
                325                 330                 335     


Lys His Arg Lys Gln Gln Val Leu Glu Thr Val Ala Gly Lys Arg Ser 
            340                 345                 350         


Tyr Arg Leu Ser Met Lys Val Lys Ala Phe Pro Ser Pro Glu Val Val 
        355                 360                 365             


Trp Leu Lys Asp Gly Leu Pro Ala Thr Glu Lys Ser Ala Arg Tyr Leu 
    370                 375                 380                 


Thr Arg Gly Tyr Ser Leu Ile Ile Lys Asp Val Thr Glu Glu Asp Ala 
385                 390                 395                 400 


Gly Asn Tyr Thr Ile Leu Leu Ser Ile Lys Gln Ser Asn Val Phe Lys 
                405                 410                 415     


Asn Leu Thr Ala Thr Leu Ile Val Asn Val Lys Pro Gln Ile Tyr Glu 
            420                 425                 430         


Lys Ala Val Ser Ser Phe Pro Asp Pro Ala Leu Tyr Pro Leu Gly Ser 
        435                 440                 445             


Arg Gln Ile Leu Thr Cys Thr Ala Tyr Gly Ile Pro Gln Pro Thr Ile 
    450                 455                 460                 


Lys Trp Phe Trp His Pro Cys Asn His Asn His Ser Glu Ala Arg Cys 
465                 470                 475                 480 


Asp Phe Cys Ser Asn Asn Glu Glu Ser Phe Ile Leu Asp Ala Asp Ser 
                485                 490                 495     


Asn Met Gly Asn Arg Ile Glu Ser Ile Thr Gln Arg Met Ala Ile Ile 
            500                 505                 510         


Glu Gly Lys Asn Lys Met Ala Ser Thr Leu Val Val Ala Asp Ser Arg 
        515                 520                 525             


Ile Ser Gly Ile Tyr Ile Cys Ile Ala Ser Asn Lys Val Gly Thr Val 
    530                 535                 540                 


Gly Arg Asn Ile Ser Phe Tyr Ile Thr Asp Val Pro Asn Gly Phe His 
545                 550                 555                 560 


Val Asn Leu Glu Lys Met Pro Thr Glu Gly Glu Asp Leu Lys Leu Ser 
                565                 570                 575     


Cys Thr Val Asn Lys Phe Leu Tyr Arg Asp Val Thr Trp Ile Leu Leu 
            580                 585                 590         


Arg Thr Val Asn Asn Arg Thr Met His Tyr Ser Ile Ser Lys Gln Lys 
        595                 600                 605             


Met Ala Ile Thr Lys Glu His Ser Ile Thr Leu Asn Leu Thr Ile Met 
    610                 615                 620                 


Asn Val Ser Leu Gln Asp Ser Gly Thr Tyr Ala Cys Arg Ala Arg Asn 
625                 630                 635                 640 


Val Tyr Thr Gly Glu Glu Ile Leu Gln Lys Lys Glu Ile Thr Ile Arg 
                645                 650                 655     


Gly Glu His Cys Asn Lys Lys Ala Val Phe Ser Arg Ile Ser Lys Phe 
            660                 665                 670         


Lys Ser Thr Arg Asn Asp Cys Thr Thr Gln Ser Asn Val Lys His 
        675                 680                 685         


<210>  2
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A4 peptide

<400>  2

Thr Glu Gly Arg Glu Leu Val Ile Pro Ala Arg Val Thr 
1               5                   10              


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B3 peptide

<400>  3

Ile Pro Ala Arg Val Thr Ser 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScrB3=S3 peptide

<400>  4

Ile Ala Arg Val Ser Thr Pro 
1               5           


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C1 peptide

<400>  5

Arg Val Thr Ser 
1               


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 peptide

<400>  6

Lys Phe Pro Leu Asp Thr Leu Ile Pro Asp Gly 
1               5                   10      


<210>  7
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A2 peptide

<400>  7

Asp Thr Leu Ile Pro Asp Gly Lys Arg Ile Ile 
1               5                   10      


<210>  8
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A3 peptide

<400>  8

Gly Arg Pro Phe Val Glu Met Tyr Ser Glu 
1               5                   10  


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A5 peptide

<400>  9

Asp Ser Arg Lys Gly Phe Ile Ile Ser Asn Ala Thr 
1               5                   10          


<210>  10
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A6 peptide

<400>  10

Glu Ala Thr Val Asn Gly His Leu Tyr Lys Thr 
1               5                   10      


<210>  11
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A7 peptide

<400>  11

Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu 
1               5                   10      


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P1 peptide

<400>  12

Ala Pro Ala Arg Val Thr Ser 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2 peptide

<400>  13

Ile Ala Ala Arg Val Thr Ser 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P3 peptide

<400>  14

Ile Pro Ala Ala Val Thr Ser 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P4 peptide

<400>  15

Ile Pro Ala Arg Ala Thr Ser 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P5 peptide

<400>  16

Ile Pro Ala Arg Val Ala Ser 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P6 peptide

<400>  17

Ile Pro Ala Arg Val Thr Ala 
1               5           


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
gcaccaagag cgatgtgtgg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
acaccacgga gttgtagtct                                                   20


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
atttcccctg ctcgtaccac t                                                 21


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
tcacgggtgg ggttcctca                                                    19


<210>  22
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  heavy chain D16F7


<220>
<221>  CDS
<222>  (1)..(399)

<400>  22
atg gga tgg agc tct atc atc ctc ttc ttg gta gca aca gcc tca ggt         48
Met Gly Trp Ser Ser Ile Ile Leu Phe Leu Val Ala Thr Ala Ser Gly           
1               5                   10                  15                

gtc cac tcc cag gtc caa ctg cag caa cct ggg tct gaa ctg gtg agg         96
Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ser Glu Leu Val Arg           
            20                  25                  30                    

cct gga gct tca gtg aag ctg tcc tgc aag gct tct ggc tac ata ttc        144
Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ile Phe           
        35                  40                  45                        

acc aac tac tgg atg cac tgg gtg aag cag agg cct gga caa ggc ctt        192
Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu           
    50                  55                  60                            

gag tgg att gga gaa att tat cct cgt aat ggt aat act aac tac gat        240
Glu Trp Ile Gly Glu Ile Tyr Pro Arg Asn Gly Asn Thr Asn Tyr Asp           
65                  70                  75                  80            

gag aag ttc aag ggc aag gcc aca ctg act ata gac aca tcc tcc agc        288
Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser           
                85                  90                  95                

aca gcc tac atg cag ctc agc agc ctg aca tct gag gac tct gcg gtc        336
Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val           
            100                 105                 110                   

tat tac tgt gcc acc tac cta ggg gac tac tgg ggc caa ggc acc act        384
Tyr Tyr Cys Ala Thr Tyr Leu Gly Asp Tyr Trp Gly Gln Gly Thr Thr           
        115                 120                 125                       

ctc aca gtc tcc tca                                                    399
Leu Thr Val Ser Ser                                                       
    130                                                                   


<210>  23
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23

Met Gly Trp Ser Ser Ile Ile Leu Phe Leu Val Ala Thr Ala Ser Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ser Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ile Phe 
        35                  40                  45              


Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Tyr Pro Arg Asn Gly Asn Thr Asn Tyr Asp 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ile Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Tyr Leu Gly Asp Tyr Trp Gly Gln Gly Thr Thr 
        115                 120                 125             


Leu Thr Val Ser Ser 
    130             


<210>  24
<211>  393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D16F7 light chain


<220>
<221>  CDS
<222>  (1)..(393)

<400>  24
atg aag ttg cct gtt agg ctg ttg gtg ctg atg ttc tgg att cct gct         48
Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala           
1               5                   10                  15                

tcc agc agt gat gtt ttg atg acc caa act cca ctc tcc ctg cct gtc         96
Ser Ser Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val           
            20                  25                  30                    

agc ctt gga gat caa gcc tcc atc tct tgc aga tct agt cag agt att        144
Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile           
        35                  40                  45                        

gtt cat agt aat gga aac acc tat tta gaa tgg ttc ctg cag aaa cca        192
Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Phe Leu Gln Lys Pro           
    50                  55                  60                            

ggc cag tct cca aag ctc ctg atc tac aaa gtt tcc aac cga ttt tct        240
Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser           
65                  70                  75                  80            

ggg atc cca gac agg ttc agt ggc agt gga tca ggg aca gat ttc aca        288
Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr           
                85                  90                  95                

ctc aag atc agt aga gtg gag gct gag gat ctg gga gtt tat ttc tgc        336
Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys           
            100                 105                 110                   

ttt caa ggt tca cat gtt ccg tac acg ttc gga ggg ggg acc aag ctg        384
Phe Gln Gly Ser His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu           
        115                 120                 125                       

gaa ata aaa                                                            393
Glu Ile Lys                                                               
    130                                                                   


<210>  25
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  25

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Phe Leu Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys 
            100                 105                 110         


Phe Gln Gly Ser His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys 
    130     


